Functional genomic analysis of Hawaii marine metagenomes


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发布日期2017-09-22
资源类型报告
全部作者Xiaoqi Wang • Qi Wang • Xiao Guo • Luying Liu • Jiangtao Guo • Jinxian Yao • Huaiqiu Zhu
机构团队北京大学自然保护与社会发展研究中心
关键字Marine microorganism Metagenome Gene annotation Codon usage bias Metabolic pathway
引用方式Wang X, Wang Q, Guo X, et al. Functional genomic analysis of Hawaii marine metagenomes[J]. Science Bulletin, 2015, 60(3):348-355.
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摘要

Using high-throughput sequencing on metage- nome to analyze marine microbial community, it is one of current main issues in the field of environmental microbe research. In this paper, we conducted the functional ana- lysis on seven samples of metagenomic data from different depth seawater in Hawaii. The results of gene prediction and function annotation indicate that there are large amounts of potential novel genes of which functions remain unknown at present. Based on the gene annotation, codon usage bias is studied on ribosomal protein-related genes and shows an evident influence by the marine extreme environment. Furthermore, focusing on the marine environmental differences such as light intensity, dissolved oxygen, temperature and pressure among various depths, comparative analysis is carried out on related genes and metabolic pathways. Thus, the understanding as well as new insights into the correlation between marine environ- ment and microbes are proposed at molecular level. Therefore, the studies herein afford a clue to reveal the special living strategies of microbial community from sea surface to deep sea.


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